Reference number: OPUSeJ 201302091731OHO
Links: to published article: http://www.ncbi.nlm.nih.gov/pubmed/23256527
to pre-reviewed version: http://www.opusej.org/wp-content/uploads/2013/03/OPUSeJ-201302091731OHOpdf.pdf
Title: Oral administration of hyaluronan reduces bone turnover in ovariectomized rats
Authors: Ma, Jenny1, Patrick V. Granton2, David W. Holdsworth2, and Eva A. Turley1*
Abstract: The effect of oral hyaluronan (HA) on bone loss in ovariectomized (OVX) 3-month-old rats was measured using serum markers of bone turnover and bone mineral density. OVX rats were administered 1 mg/kg HA (OVX + HA) or phosphate-buffered saline (PBS) (OVX + PBS) by oral gavage (5 days/week for 54 days). Additional controls included sham ovariectomy with PBS gavage (Sham + PBS) and no treatment. Oral administration of HA resulted in approximately 50% (p < 0.05) increases in serum HA. Gel filtration analyses showed this was high molecular weight HA (300-500 kDa). Osteopenia was mild due to the young age of the animals. Thus, ovariectomy resulted in a 30% increase in serum collagen N-terminal telopeptides (p < 0.001), a 20% increase in serum nitrate/nitrite levels (p = 0.05), and a 5-6% decrease in femur bone mineral density/content (p < 0.05). HA gavage blunted the development of osteopenia in this model as determined by preventing the 30% increase in serum collagen N-terminal telopeptide levels (p < 0.001) and by reducing bone mineral content loss from 6 to 4%. These results show that oral supplements of HA (gavage solution, 0.12% solution) significantly reduce bone turnover associated with mild osteopenia in rats.
Author bio: N/A
Sponsor editor: N/A
Affiliations/disclaimers/funding/acknowledgements: 1 London Regional Cancer Program, London Health Sciences Center, Victoria Hospital; Department of Biochemistry and Oncology, University of Western Ontario, London, Ontario, Canada. 2 Imaging Research Laboratories, Robarts Research Institute, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario Canada. *Postal address: London Regional Cancer Program, London Health Sciences Center, Victoria Hospital; Dept. of Biochemistry and Oncology, Western University, Victoria Hospital, 790 Commissioners Road E., London, ON, Canada N6A 4L6. E-mail: email@example.com. Phone: 1 (519) 685-8600. Fax: 1 (519) 685-8616.
Keywords: hyaluronan; hyaluronic acid; bone turnover; oral gavage; osteopenia; ovariectomized rats
Subject: Science/ osteopenia
Bibliography: (alphabetical) N/A
Citation: Ma J, et al, 2013, “Oral administration of hyaluronan reduces bone turnover in ovariectomized rats”, J Agric Food Chem. 16:61(2). 339-45. doi: 10.1021/jf300651d. Epub 2013 Jan 8. http://www.ncbi.nlm.nih.gov/pubmed/23256527